TRE-dCas9(sa)-VP64-AAV
(Plasmid
#213035)
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PurposedCas9-VP64 expressed under control of TRE
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213035 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
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SerotypeSelect serotype for details See details about
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PricingSelect serotype and quantity $ USD for preparation of µL virus + $32 USD for plasmid.
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Backbone
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Vector backboneAAV
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Vector typeAAV, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namedCas9
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SpeciesStaphylococcus aureus
- Promoter CMV
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Tags
/ Fusion Proteins
- NLS (N terminal on insert)
- NLS-VP64 (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.03.28.534564 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TRE-dCas9(sa)-VP64-AAV was a gift from Nadav Ahituv (Addgene plasmid # 213035 ; http://n2t.net/addgene:213035 ; RRID:Addgene_213035) -
For your References section:
Implantation of engineered adipocytes suppresses tumor progression in cancer models. Nguyen HP, An K, Ito Y, Kharbikar BN, Sheng R, Paredes B, Murray E, Pham K, Bruck M, Zhou X, Biellak C, Ushiki A, Nobuhara M, Fong SL, Bernards DA, Lynce F, Dillon DA, Magbanua MJM, Huppert LA, Hammerlindl H, Klein JA, Valdiviez L, Fiehn O, Esserman L, Desai TA, Yee SW, Rosenbluth JM, Ahituv N. Nat Biotechnol. 2025 Feb 4:10.1038/s41587-024-02551-2. doi: 10.1038/s41587-024-02551-2. 10.1038/s41587-024-02551-2 PubMed 39905264