pAAV-GG
(Plasmid
#213039)
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Purpose(Empty Backbone) AAV backbone for Golden Gate assembly of donor templates using BbsI/BpiI (overhangs AGCG and GCAA, respectively)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 213039 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepAAV-MCS
- Backbone size (bp) 4650
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Modifications to backboneInserted BbsI-flanked stuffer sequence
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Vector typeAAV ; vector for Golden gate assembly
- Promoter N/A
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Tag
/ Fusion Protein
- N/A
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-GG was a gift from Fredrik Sterky (Addgene plasmid # 213039 ; http://n2t.net/addgene:213039 ; RRID:Addgene_213039) -
For your References section:
Efficient Gene-Editing in Human Pluripotent Stem Cells Through Simplified Assembly of Adeno-Associated Viral (AAV) Donor Templates. De La Cruz BM, Mitra S, He B, Celik M, Kaminski D, Smedler E, Sterky FH. Bio Protoc. 2024 Nov 5;14(21):e5097. doi: 10.21769/BioProtoc.5097. eCollection 2024 Nov 5. 10.21769/BioProtoc.5097 PubMed 39525974
Map uploaded by the depositor.
