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PurposeExpresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.
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Depositing Lab
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Depositing OrganizationStanford University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 213777 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 213777-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 213777-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonePiggyBac
- Total vector size (bp) 17168
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Vector typeCRISPR
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameVPR-dSaCas9
- Promoter Tet_On_TRE
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer NA
- 3′ sequencing primer NA
- (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namedSpCas9-KRAB-BFP
- Promoter Tet_On_TRE
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer NA
- (Common Sequencing Primers)
Gene/Insert 3
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Gene/Insert namezeocin
- Promoter EF1a (EF-1-alpha intron a)
Cloning Information for Gene/Insert 3
- Cloning method Unknown
- 5′ sequencing primer NA
- (Common Sequencing Primers)
Gene/Insert 4
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Gene/Insert nameTet-On transactivator
- Promoter EF1a (EF-1-alpha intron a)
Cloning Information for Gene/Insert 4
- Cloning method Unknown
- 5′ sequencing primer NA
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 213777-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 213777-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p101_CRISPRai_PiggyBac was a gift from Howard Chang (Addgene plasmid # 213777 ; http://n2t.net/addgene:213777 ; RRID:Addgene_213777) -
For your References section:
Bidirectional epigenetic editing reveals hierarchies in gene regulation. Pacalin NM, Steinhart Z, Shi Q, Belk JA, Dorovskyi D, Kraft K, Parker KR, Shy BR, Marson A, Chang HY. Nat Biotechnol. 2024 May 17. doi: 10.1038/s41587-024-02213-3. 10.1038/s41587-024-02213-3 PubMed 38760566
Map uploaded by the depositor.
