AAVS1-eTLR
(Plasmid
#214737)
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Purposeencodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locus
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Depositing Lab
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Depositing OrganizationHelmholtz Munich
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214737 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 214737-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 214737-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBlueScript
- Backbone size w/o insert (bp) 3948
- Total vector size (bp) 11147
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Vector typeMammalian Expression, CRISPR, Synthetic Biology
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAAVS1-eTLR
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SpeciesH. sapiens (human), G. gallus (chicken), B. taurus (bovine), O. cuniculus (rabbit), Synthetic; CMV, Anguilla japonica, influenza
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Insert Size (bp)7199
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Entrez GenePPP1R12C (a.k.a. AAVS1, LENG3, MBS85, p84, p85)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 214737-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 214737-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAVS1-eTLR was a gift from Gil Westmeyer (Addgene plasmid # 214737 ; http://n2t.net/addgene:214737 ; RRID:Addgene_214737) -
For your References section:
Exonuclease-enhanced prime editors. Truong DJ, Geilenkeuser J, Wendel SV, Wilming JCH, Armbrust N, Binder EMH, Santl TH, Siebenhaar A, Gruber C, Phlairaharn T, Zivanic M, Westmeyer GG. Nat Methods. 2024 Feb 1. doi: 10.1038/s41592-023-02162-w. 10.1038/s41592-023-02162-w PubMed 38302659
