pCMV_RTN3E-mScarlet3
(Plasmid
#216255)
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Purposemammalian expression vector for RTN3E-mScarlet3
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Depositing Lab
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Depositing OrganizationUniversity of Cambridge
Located in the United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216255 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 216255-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 216255-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCMV
- Backbone size w/o insert (bp) 4672
- Total vector size (bp) 7766
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namereticulon 3 isoform e
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Alt nameRTN3E
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SpeciesH. sapiens (human)
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Insert Size (bp)3096
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GenBank IDNM_001265589
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Entrez GeneRTN3 (a.k.a. ASYIP, HAP, NSPL2, NSPLII, RTN3-A1)
- Promoter CMV
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Tag
/ Fusion Protein
- mScarlet3 (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer -
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 216255-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 216255-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV_RTN3E-mScarlet3 was a gift from Edward Avezov (Addgene plasmid # 216255 ; http://n2t.net/addgene:216255 ; RRID:Addgene_216255) -
For your References section:
Luminal transport through intact endoplasmic reticulum limits the magnitude of localized Ca(2+) signals. Crapart CC, Scott ZC, Konno T, Sharma A, Parutto P, Bailey DMD, Westrate LM, Avezov E, Koslover EF. Proc Natl Acad Sci U S A. 2024 Mar 26;121(13):e2312172121. doi: 10.1073/pnas.2312172121. Epub 2024 Mar 19. 10.1073/pnas.2312172121 PubMed 38502705
