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pcDNA3.1-HaloAKAR2.1-NES-T2A-EGFP-CAAX
(Plasmid #216491)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216491 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HaloAKAR2.1
  • Species
    Synthetic
  • Promoter CMV
  • Tag / Fusion Protein
    • NES, T2A-EGFP-CAAX (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-HaloAKAR2.1-NES-T2A-EGFP-CAAX was a gift from Jin Zhang (Addgene plasmid # 216491 ; http://n2t.net/addgene:216491 ; RRID:Addgene_216491)

  • For your References section:

    Far-red chemigenetic kinase biosensors enable multiplexed and super-resolved imaging of signaling networks. Frei MS, Sanchez SA, He X, Liu L, Schneider F, Wang Z, Hakozaki H, Li Y, Lyons AC, Rohm TV, Olefsky JM, Shi L, Schoneberg J, Fraser SE, Mehta S, Wang Y, Zhang J. Nat Biotechnol. 2025 Apr 21. doi: 10.1038/s41587-025-02642-8. 10.1038/s41587-025-02642-8 PubMed 40258957
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