mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT
(Plasmid
#216763)
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PurposePiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase
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Depositing Lab
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Depositing OrganizationStanford University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 216763 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 216763-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 216763-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonePiggyBac
- Backbone size w/o insert (bp) 7600
- Total vector size (bp) 13200
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
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SpeciesG. gallus (chicken), Synthetic
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Insert Size (bp)5600
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Tags
/ Fusion Proteins
- mCherry (N terminal on backbone)
- EGFP (N terminal on backbone)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This PILATeS module contains BiT tag with 700 pM affinity for LgBiT. PILATeS is appended C-terminally to vinculin as an unloaded negative control. Requires co-transfection with transposase
DNA (Catalog # 216763-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 216763-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT was a gift from Alex Dunn (Addgene plasmid # 216763 ; http://n2t.net/addgene:216763 ; RRID:Addgene_216763) -
For your References section:
Split Luciferase Molecular Tension Sensors for Bioluminescent Readout of Mechanical Forces in Biological Systems. Zhong BL, Elliot JM, Wang P, Li H, Hall RN, Wang B, Prakash M, Dunn AR. ACS Sens. 2024 Jul 26;9(7):3489-3495. doi: 10.1021/acssensors.3c02664. Epub 2024 Jul 7. 10.1021/acssensors.3c02664 PubMed 38973210
Map uploaded by the depositor.
