pAAV-ITR-CMV-mCherry-1xU6-LtR-wt(TAG)
(Plasmid #217366)

• Purpose: Expresses E. coli leucine tRNA and a wild-type mCherry reporter; can be packaged into AAV
• Depositing Lab: Abhishek Chatterjee
• Publication: Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2149. doi: 10.1021/acssynbio.4c00196. Epub 2024 Jun 21.

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Backbone

• Vector backbone: pAAV-ITR-mCherry
• Backbone size w/o insert (bp): 5498
• Total vector size (bp): 5854
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: E. coli leucine tRNA for TAG suppression
• Alt name: LtR
• Alt name: tRNA-Leu
• Alt name: EcLtR
• Species: E. coli
• Insert Size (bp): 356
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: CTGCGGCCGCACGCGTCTCGCGGTCCAG
• 3′ sequencing primer: GCTGCTCTCGTCGATCCGCTAGC

Resource Information

• Supplemental Documents:
  • paav-itr-cmv-mcherry-1xu6-ltr-wttag.gb
• A portion of this plasmid was derived from a plasmid made by: from Wu N, Deiters A, Cropp TA, King D, Schultz PG. A genetically encoded photocaged amino acid. J Am Chem Soc. 2004 Nov 10;126(44):14306-7. doi: 10.1021/ja040175z. PMID: 15521721.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry