MZB150
(Plasmid #217840)

• Purpose: Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (52-252) of Cytohesin-2 (CYTH2/ARNO) for rapid nucleotide exchange and activation of Arf GTPases
• Depositing Lab: Richard Baker
• Publication: Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2411974121. doi: 10.1073/pnas.2411974121. Epub 2024 Dec 20.

Backbone

• Vector backbone: pET-29b(+)
• Backbone manufacturer: Novagen
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ARNO
• Alt name: Cytohesin-2
• Alt name: CYTH2
• Species: H. sapiens (human)
• Mutation: Deleted residues 1-51 and 253-400
• Entrez Gene: CYTH2 (a.k.a. ARNO, CTS18, CTS18.1, PSCD2, PSCD2L, SEC7L, Sec7p-L, Sec7p-like, cytohesin-2)
• Tags / Fusion Proteins:
  • 10x His (N terminal on insert)
  • SUMO (SMT3) (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Terminator

Resource Information

• Supplemental Documents:
  • MZB150_pLann.gb
• A portion of this plasmid was derived from a plasmid made by: James Hurley, PhD- Department of Molecular & Cell Biology University of California, Berkeley

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry