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Addgene

pb-CAGGS-dCas9-blasto
(Plasmid #218555)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218555 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    PiggyBac transposon
  • Vector type
    Mammalian Expression, CRISPR ; PiggyBac transposition
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9
  • Species
    S. pyogenes
  • Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N863A
  • Tag / Fusion Protein
    • 2xSV40NLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pb-CAGGS-dCas9-blasto was a gift from Alejandro Chavez (Addgene plasmid # 218555 ; http://n2t.net/addgene:218555 ; RRID:Addgene_218555)
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