pGP-CAG-iGABASnFR2n-WPRE-bGH-polyA
(Plasmid
#218869)
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PurposeMammalian expression of improved GABA sensor (negative change in fluorescence)
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Depositing Lab
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Depositing OrganizationHHMI, Janelia Research Campus
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 218869 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 218869-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 218869-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameiGABASnFR2n
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Alt namevariant 4445
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SpeciesPseudomonas fluorescens, A. victoria (jellyfish)
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Insert Size (bp)1941
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MutationS99A F104H R168P
- Promoter CAG
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.03.25.644953 for bioRxiv preprint.
DNA (Catalog # 218869-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 218869-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGP-CAG-iGABASnFR2n-WPRE-bGH-polyA was a gift from GENIE Project (Addgene plasmid # 218869 ; http://n2t.net/addgene:218869 ; RRID:Addgene_218869) -
For your References section:
iGABASnFR2 is an improved genetically encoded protein sensor of GABA. Kolb I, Hasseman JP, Matsumoto A, Jensen TP, Kopach O, Arthur BJ, Zhang Y, Tsang A, Reep D, Tsegaye G, Zheng J, Patel RH, Looger LL, Marvin JS, Korff WL, Rusakov DA, Yonehara K, Turner GC. eLife. 2026 Mar 18;14:RP108319. doi: 10.7554/eLife.108319. 10.7554/eLife.108319 PubMed 41848771
