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Addgene

CRY2olig-PR
(Plasmid #218917)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218917 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    CRY2olig-mCherry #60032
  • Backbone size w/o insert (bp) 6188
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRY2olig-mCherry tagged poly-PR
  • Species
    H. sapiens (human)
  • Mutation
    wild-type
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrGI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CRY2olig-PR was a gift from Tatyana Shelkovnikova (Addgene plasmid # 218917 ; http://n2t.net/addgene:218917 ; RRID:Addgene_218917)

  • For your References section:

    C9orf72 poly-PR forms anisotropic condensates causative of nuclear TDP-43 pathology. Hodgson RE, Rayment JA, Huang WP, Sanchez Avila A, Ellis BCS, Lin YH, Soni N, Hautbergue GM, Shelkovnikova TA. iScience. 2024 Sep 14;27(10):110937. doi: 10.1016/j.isci.2024.110937. eCollection 2024 Oct 18. 10.1016/j.isci.2024.110937 PubMed 39391721
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