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(Plasmid #21899)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 21899 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    needs P3-containing host strain such as DH10B/P3
  • Copy number


  • Gene/Insert name
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Ddit3 (a.k.a. AltDDIT3, CHOP-, CHOP-10, CHOP10, ch, chop, gadd15, gadd153)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

Note that the phagemid had a scrambled insert and the portion 3' of the CHOP-10 coding region (in red-see author's map) is not from the CHOP gene. If you wish to obtain a CHOP probe for hybridization experiments we suggest excising the HD3-Nhe1 fragment that contains CHOP coding sequence.

Note that this plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCHOP10.pCDNA1 was a gift from David Ron (Addgene plasmid # 21899 ; ; RRID:Addgene_21899)