|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21899||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Tetracycline
Growth instructionsneeds P3-containing host strain such as DH10B/P3
SpeciesM. musculus (mouse)
Entrez GeneDdit3 (a.k.a. AltDDIT3, CHOP-, CHOP-10, CHOP10, ch, chop, gadd15, gadd153)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Note that the phagemid had a scrambled insert and the portion 3' of the CHOP-10 coding region (in red-see author's map) is not from the CHOP gene. If you wish to obtain a CHOP probe for hybridization experiments we suggest excising the HD3-Nhe1 fragment that contains CHOP coding sequence.
Note that this plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mCHOP10.pCDNA1 was a gift from David Ron (Addgene plasmid # 21899 ; http://n2t.net/addgene:21899 ; RRID:Addgene_21899)
Map uploaded by the depositor.