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pLVX-EF1a-mito-mRaspberry
(Plasmid #219568)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219568 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLVX
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 7462
  • Total vector size (bp) 8251
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mito
  • Alt name
    COX8A presequence
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    72
  • GenBank ID
    NM_004074.3
  • Entrez Gene
    COX8A (a.k.a. COX, COX8, COX8-2, COX8L, MC4DN15, VIII, VIII-L)
  • Promoter EF1a
  • Tag / Fusion Protein
    • mRaspberry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI-HF (not destroyed)
  • 3′ cloning site BsrgII-HF (not destroyed)
  • 5′ sequencing primer GCGGCCGCATGTCCGTCCTGACGCCGC
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    mito-mRaspberry was amplified from Addgene plasmid #55931. The vector backbone is pLVX from clontech.

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-EF1a-mito-mRaspberry was a gift from Thomas Schwarz (Addgene plasmid # 219568 ; http://n2t.net/addgene:219568 ; RRID:Addgene_219568)

  • For your References section:

    Energy stress activates AMPK to arrest mitochondria via phosphorylation of TRAK1. Falk JE, Henke T, Gowrisankaran S, Wanderoy S, Basu H, Greally S, Steen J, Schwarz TL. J Cell Biol. 2026 Apr 6;225(4):e202501023. doi: 10.1083/jcb.202501023. Epub 2026 Jan 30. 10.1083/jcb.202501023 PubMed 41615403
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