pmEGFP-C1-Nir3-LNS2
(Plasmid #220087)

• Purpose: C-terminal PA binding domain of Nir3
• Depositing Lab: Gerry Hammond
• Publication: Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e202405174. doi: 10.1083/jcb.202405174. Epub 2025 Sep 9.

Backbone

• Vector backbone: pcDNA3.1-mEGFP-C1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PITPNM2
• Alt name: Nir3
• Species: H. sapiens (human)
• Insert Size (bp): 855
• Mutation: amino acids 925-1209
• Entrez Gene: PITPNM2 (a.k.a. NIR-3, NIR3, RDGB2, RDGBA2)
• Promoter: CMV
• Tag / Fusion Protein:
  • mEGFP-GGGGSHM (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BmtI (not destroyed)
• 3′ cloning site: NotI (not destroyed)

Resource Information

• Supplemental Documents:
  • pmEGFP-C1-Nir3-LNS2
• A portion of this plasmid was derived from a plasmid made by: Dr. Shujuan Gao, StonyBrook University, NY

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry