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Addgene

pHHM.X513.iS-methadoneSnFR
(Plasmid #220861)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220861 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pHHM.X513
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iS-methadoneSnFR
  • Species
    Synthetic
  • Tags / Fusion Proteins
    • His6-HA (N terminal on insert)
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHHM.X513.iS-methadoneSnFR was a gift from Henry Lester (Addgene plasmid # 220861 ; http://n2t.net/addgene:220861 ; RRID:Addgene_220861)

  • For your References section:

    Three Mutations Convert the Selectivity of a Protein Sensor from Nicotinic Agonists to S-Methadone for Use in Cells, Organelles, and Biofluids. Muthusamy AK, Kim CH, Virgil SC, Knox HJ, Marvin JS, Nichols AL, Cohen BN, Dougherty DA, Looger LL, Lester HA. J Am Chem Soc. 2022 May 18;144(19):8480-8486. doi: 10.1021/jacs.2c02323. Epub 2022 Apr 21. 10.1021/jacs.2c02323 PubMed 35446570
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