pCas9_antiGFP
(Plasmid
#220923)
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PurposeExpresses Cas9 with a GFP targeting crRNA for use in targeting the GFP plasmid in a cell based assay
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220923 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepYTK001
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namepCas9
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SpeciesSynthetic
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Insert Size (bp)4107
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThe gene was cloned by the Marraffini group. We received the original plasmid from addgene.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCas9_antiGFP was a gift from Ryan Jackson (Addgene plasmid # 220923 ; http://n2t.net/addgene:220923 ; RRID:Addgene_220923)