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mCherry-LC3
(Plasmid #221656)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 221656 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pmCherryC1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4693
  • Total vector size (bp) 5100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MAP1LC3B
  • Alt name
    LC3
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    738
  • GenBank ID
    U05784
  • Entrez Gene
    Map1lc3b (a.k.a. LC3B, Map1lc3, Mpl3, zbs559)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (unknown if destroyed)
  • 3′ cloning site Bgl2 (unknown if destroyed)
  • 5′ sequencing primer 5'-ATCGTGGAACAGTACGAACG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert cloned from EGFP-LC3B provided by Tamotsu Yoshimori, Osaka University

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-LC3 was a gift from Erika Holzbaur (Addgene plasmid # 221656 ; http://n2t.net/addgene:221656 ; RRID:Addgene_221656)

  • For your References section:

    Mitochondrial damage triggers the concerted degradation of negative regulators of neuronal autophagy. Basak B, Holzbaur ELF. Nat Commun. 2025 Aug 9;16(1):7367. doi: 10.1038/s41467-025-62379-5. 10.1038/s41467-025-62379-5 PubMed 40783388
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