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Addgene

pSWITCH
(Plasmid #222402)

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Item Catalog # Description Quantity Price (USD)
Plasmid 222402 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pFS
  • Backbone size (bp) 4571
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSWITCH was a gift from Anne Botteaux (Addgene plasmid # 222402 ; http://n2t.net/addgene:222402 ; RRID:Addgene_222402)

  • For your References section:

    Efficient and rapid one-step method to generate gene deletions in Streptococcus pyogenes. Schiavolin L, Lakhloufi D, Botquin G, Deneubourg G, Bruyns C, Steinmetz J, Henrot C, Delforge V, Smeesters PR, Botteaux A. Microbiol Spectr. 2024 Oct 3;12(10):e0118524. doi: 10.1128/spectrum.01185-24. Epub 2024 Aug 20. 10.1128/spectrum.01185-24 PubMed 39162539
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