Skip to main content
Addgene

pSF8
(Plasmid #222592)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 222592 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    BB2_L_AB_syn_BbsI
  • Backbone manufacturer
    Michael Sauer (Sarkari et al. 2017) Addgene #89917
  • Total vector size (bp) 7595
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gsdA
  • Species
    Aspergillus niger ATCC 1015
  • Insert Size (bp)
    2364
  • Mutation
    Internal BsaI and BpiI sites removed from insert, PAM site of gRNA1(gsdA) removed
  • Promoter tet-on

Cloning Information

  • Cloning method Golden Gate
  • 5′ sequencing primer CATTAATGCAGCTGGCAC
  • 3′ sequencing primer GGTTATTGTCTCATGAGCGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSF8 was a gift from Matthias Steiger (Addgene plasmid # 222592 ; http://n2t.net/addgene:222592 ; RRID:Addgene_222592)

  • For your References section:

    Regulation of the Glucose-6-phosphate dehydrogenase encoding gene gsdA and its impact on growth and citric acid production in Aspergillus niger. Matthias Steiger. PLOS One 10.1371/journal.pone.0321363
Related items: