pAAV-hSyn-fDIO-hM3D(Gq)-mCherry
(Plasmid #223652)

• Purpose: Expresses hM3D(Gq) in a flippase dependent manner
• Depositing Lab: Oscar Marin
• Publication: Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586-025-08933-z.

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4954
• Total vector size (bp): 7489
• Modifications to backbone: Replaced DIO cassette with fDIO (FRT) casette
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hM3D(Gq)-mCherry
• Species: H. sapiens (human)
• Insert Size (bp): 2522
• Promoter: hSyn
• Tag / Fusion Protein:
  • mCherry

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pAAV-hSyn-fDIO-hM3D(Gq)-mCherry.ape
• A portion of this plasmid was derived from a plasmid made by: Bryan Roth

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.02.03.527010 for bioRxiv preprint.