pAAV-hSyn-fDIO-hM3D(Gq)-mCherry
(Plasmid
#223652)
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PurposeExpresses hM3D(Gq) in a flippase dependent manner
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 223652 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
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SerotypeSelect serotype for details See details about
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PricingSelect serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
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Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 4954
- Total vector size (bp) 7489
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Modifications to backboneReplaced DIO cassette with fDIO (FRT) casette
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehM3D(Gq)-mCherry
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SpeciesH. sapiens (human)
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Insert Size (bp)2522
- Promoter hSyn
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Tag
/ Fusion Protein
- mCherry
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byBryan Roth
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.02.03.527010 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-hSyn-fDIO-hM3D(Gq)-mCherry was a gift from Oscar Marin (Addgene plasmid # 223652 ; http://n2t.net/addgene:223652 ; RRID:Addgene_223652) -
For your References section:
Regulation of PV interneuron plasticity by neuropeptide-encoding genes. Selten M, Bernard C, Mukherjee D, Hamid F, Hanusz-Godoy A, Oozeer F, Zimmer C, Marín O. Nature 2025.04.30 10.1038/s41586-025-08933-z