pAAV-hSyn-DIO-hM3D(Gq)-mCherry-shLacZ-CWB
(Plasmid #223660)

• Purpose: Expresses hM3D(Gq) and a control shRNA (targetting LacZ) in a Cre-dependent manner
• Depositing Lab: Oscar Marin
• Publication: Selten et al Nature 2025.04.30

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Backbone

• Vector backbone: pAAV
• Backbone manufacturer: Beatriz Rico
• Backbone size w/o insert (bp): 4290
• Total vector size (bp): 7403
• Modifications to backbone: Added CWB cassette from Bong-Kiun Kaang
• Vector type: Mammalian Expression, AAV, RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hM3D(Gq)-mCherry and shLacZ
• Species: H. sapiens (human)
• Insert Size (bp): 3143
• Promoter: hSyn
• Tag / Fusion Protein:
  • mCherry

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pAAV-hSyn-DIO-hM3D(Gq)-mCherry-shLacZ-CWB.embl
• A portion of this plasmid was derived from a plasmid made by: Beatriz Rico, Bryan Roth, Bong-Kiun Kaang

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.02.03.527010 for bioRxiv preprint.