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pEN243-EN1953
(Plasmid #224546)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 224546 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pX459
  • Backbone manufacturer
    Addgene #62988
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas9-2A-puro and sgRNA targeting the first coding ATG of mouse Nipbl
  • gRNA/shRNA sequence
    CCAGAACTTCAGGATGAATG
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Nipbl (a.k.a. Idn3)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEN243-EN1953 was a gift from Elphege Nora (Addgene plasmid # 224546 ; http://n2t.net/addgene:224546 ; RRID:Addgene_224546)

  • For your References section:

    Synergy between regulatory elements can render cohesin dispensable for distal enhancer function. Hansen KL, Adachi AS, Braccioli L, Kadvani S, Boileau RM, Martinovic M, Pokorny B, Shah R, Anderson EC, Zhang K, Carel I, Bonitto K, Blelloch R, Fudenberg G, de Wit E, Nora EP. Science. 2025 Nov 27:eadt4221. doi: 10.1126/science.adt4221. 10.1126/science.adt4221 PubMed 41308125
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