pcDNA3.1-HA-GTSE1
(Plasmid
#224719)
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PurposeFor expression of HA-tagged GTSE1 protein
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Depositing Lab
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Depositing OrganizationNYU Grossman School of Medicine
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 224719 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 224719-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 224719-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGTSE1
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Alt nameB99
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SpeciesH. sapiens (human)
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Insert Size (bp)2200
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Entrez GeneGTSE1 (a.k.a. B99)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV Forward
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.06.26.600797 for bioRxiv preprint.
DNA (Catalog # 224719-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 224719-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-HA-GTSE1 was a gift from Michele Pagano (Addgene plasmid # 224719 ; http://n2t.net/addgene:224719 ; RRID:Addgene_224719) -
For your References section:
Stabilization of GTSE1 by cyclin D1-CDK4/6-mediated phosphorylation promotes cell proliferation: relevance in cancer prognosis. Garcia-Vazquez N, Gonzalez-Robles TJ, Lane E, Spasskaya D, Zhang Q, Kerzhnerman M, Jeong Y, Collu M, Simoneschi D, Ruggles KV, Rona G, Kaisari S, Pagano M. bioRxiv [Preprint]. 2025 Jan 23:2024.06.26.600797. doi: 10.1101/2024.06.26.600797. 10.1101/2024.06.26.600797 PubMed 38979260
