pBS L30 ALFA-Apex-Rab7A
(Plasmid
#224892)
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PurposeRab7A fused to APEX and an ALFA tag on N-term in a pBS L30 backbone.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 224892 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepBS L30 backbone
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRab7A
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SpeciesH. sapiens (human)
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GenBank ID
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Entrez GeneRAB7A (a.k.a. CMT2B, PRO2706, RAB7)
- Promoter Gibson Cloning
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Tags
/ Fusion Proteins
- ALFA (N terminal on insert)
- APEX2 (N terminal on insert)
Cloning Information
- Cloning method Other
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.10.07.680694 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS L30 ALFA-Apex-Rab7A was a gift from Raphael Gaudin (Addgene plasmid # 224892 ; http://n2t.net/addgene:224892 ; RRID:Addgene_224892) -
For your References section:
Monitoring the dynamics of exosomes through the tracking of Rab7 partners. Bare Y, Delalande F, Hirschler A, Cazevieille C, Lucansky V, Carapito C, Gaudin R, Deffieu MS. bioRxiv 2025.10.07.680694 10.1101/2025.10.07.680694