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Addgene

pHAGE-mCherry-CLCN3
(Plasmid #225514)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 225514 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHAGE
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CLCN3
  • Species
    H. sapiens (human)
  • Entrez Gene
    CLCN3 (a.k.a. CLC3, ClC-3, NEDHYBA, NEDSBA)
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.02.07.636106 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-mCherry-CLCN3 was a gift from Wade Harper (Addgene plasmid # 225514 ; http://n2t.net/addgene:225514 ; RRID:Addgene_225514)

  • For your References section:

    EndoMAP.v1 charts the structural landscape of human early endosome complexes. Gonzalez-Lozano MA, Schmid EW, Miguel Whelan E, Jiang Y, Paulo JA, Walter JC, Harper JW. Nature. 2025 Jul;643(8070):252-261. doi: 10.1038/s41586-025-09059-y. Epub 2025 May 28. 10.1038/s41586-025-09059-y PubMed 40437099
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