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p-att-ef1a-ABE8e-dCas9-BSD
(Plasmid #226116)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226116 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    ABE8e (Addgene #138489)
  • Backbone manufacturer
    David Liu
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BSD
  • Alt name
    Blasticidin S deaminase
  • Species
    Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

All inserts were synthetically produced by Twist Bio. nCas9 was converted to dCas9 via a redesigned fragment.

Please visit https://doi.org/10.1101/2024.07.08.602524 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-att-ef1a-ABE8e-dCas9-BSD was a gift from George Church (Addgene plasmid # 226116 ; http://n2t.net/addgene:226116 ; RRID:Addgene_226116)

  • For your References section:

    Plasmid2MC: efficient cell-free generation of high-purity minicircle DNA for genome editing in mammalian cells. Oliynyk RT, Mahas A, Karpinski E, Church GM. Commun Biol 8 (2025). https://doi.org/10.1038/s42003-025-09157-7 10.1101/2024.07.08.602524
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