PB_gRNA_S.pyogenes_scaffold_BB
(Plasmid
#226429)
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Purpose(Empty Backbone) PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 226429 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonePB-U6insert-EF1puro
- Backbone size (bp) 6462
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Modifications to backboneinsertion of Golden Gate cloning site and S. pyogenes sgRNA scaffold sequence.
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Vector typeMammalian Expression, CRISPR ; PiggyBac Transposon
- Promoter U6
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB_gRNA_S.pyogenes_scaffold_BB was a gift from Alon Goren (Addgene plasmid # 226429 ; http://n2t.net/addgene:226429 ; RRID:Addgene_226429)
Map uploaded by the depositor.
