pAAV/mIba1.GFP.miR-9.T.miR-129-2-3p.T.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA
(Plasmid #226475)

• Purpose: Improved microglia-targeted transgene expression
• Depositing Lab: Hirokazu Hirai
• Publication: Aoki et al Cell Rep Methods. 2025 Jul 25:101116. doi: 10.1016/j.crmeth.2025.101116.

Want a viral vector made from this plasmid?

Make a packaging request and we'll get back to you.

This service is available to academics and nonprofits only.

Please log in to submit a packaging request.

• Serotype: Select serotype for details See details about
• Pricing: Select serotype and quantity $ USD for preparation of µL virus + $32 USD for plasmid.
• How this works:
  • Place a request for a quantity of 2 (0.2 mL), 10 (1 mL), 25 (2.5 mL), or 50 (5 mL). Our all-inclusive pricing includes DNA production and QC.
  • Addgene will quickly confirm that we can produce a high-quality prep for you.
  • Track your request and place an order from within your account. Payment information must be added before we can begin processing your order.
  • Receive your prep in 6–9 weeks after the MTA is approved by your organization.
  • Learn more about our Packaged on Request Service.

Backbone

• Vector backbone: pAAV
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: mIba1 promoter, 1.7-kb, microglia-specific promoter
• Alt name: mouse Iba1 promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 1678

Gene/Insert 2

• Gene/Insert name: target sequences for miR-9x4 & miR-129-2-3px4 *2
• Alt name: [miR-9.Tx4 & miR-129-2.3p.Tx4]*2
• Species: Synthetic
• Insert Size (bp): 420

Gene/Insert 3

• Gene/Insert name: EGFP
• Insert Size (bp): 720

Resource Information

• Supplemental Documents:
  • pAAV mIba1(1700) GFP miR-9-T×4 miR-129-2-3p.T×4 WPRE miR-9-T×4 miR-129-2-3p.T×4 SV40pA_Konno13,H7.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains deletions in left ITR, but we can produce AAV vector using this plasmid.

Please visit https://doi.org/10.1101/2024.11.05.622037 for bioRxiv preprint.