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pAAV/mIba1.GFP.miR-9.T.miR-129-2-3p.T.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA
(Plasmid #226475)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 226475 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    mIba1 promoter, 1.7-kb, microglia-specific promoter
  • Alt name
    mouse Iba1 promoter
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1678

Gene/Insert 2

  • Gene/Insert name
    target sequences for miR-9x4 & miR-129-2-3px4 *2
  • Alt name
    [miR-9.Tx4 & miR-129-2.3p.Tx4]*2
  • Species
    Synthetic
  • Insert Size (bp)
    420

Gene/Insert 3

  • Gene/Insert name
    EGFP
  • Insert Size (bp)
    720

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains deletions in left ITR, but we can produce AAV vector using this plasmid.

Please visit https://doi.org/10.1101/2024.11.05.622037 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV/mIba1.GFP.miR-9.T.miR-129-2-3p.T.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA was a gift from Hirokazu Hirai (Addgene plasmid # 226475 ; http://n2t.net/addgene:226475 ; RRID:Addgene_226475)
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