AAV-pSyn_R-PTEN
(Plasmid
#227437)
-
PurposeExpression of the R-PTEN sensor under the Synapsin promoter in an AAV backbone
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 227437 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneAAV pSyn
-
Vector typeMammalian Expression, AAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameR-PTEN sensor
-
Alt namemCyRFP2-PTEN-mMaroon
-
SpeciesR. norvegicus (rat)
-
MutationR14G
-
Entrez GenePten (a.k.a. MMAC1, Mmac, TEP1)
- Promoter Synapsin
-
Tags
/ Fusion Proteins
- mCyRFP2 (N terminal on insert)
- mMaroon (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer Syn-for (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byThe PTEN gene was obtained from Addgene plasmid #110181 pEGFP-PTEN
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.10.04.616644 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
AAV-pSyn_R-PTEN was a gift from Tal Laviv (Addgene plasmid # 227437 ; http://n2t.net/addgene:227437 ; RRID:Addgene_227437) -
For your References section:
Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brain. Kagan T, Gabay M, Meenakshisundaram A, Levi Y, Eid S, Malchenko N, Maman M, Nitzan A, Ravotto L, Zaidel-Bar R, Eickholt BJ, Gal M, Laviv T. Nat Methods. 2025 Apr;22(4):764-777. doi: 10.1038/s41592-025-02610-9. Epub 2025 Feb 20. 10.1038/s41592-025-02610-9 PubMed 39979596