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pMX-Flag-GFP_SLC45A1
(Plasmid #227951)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 227951 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pMX-Flag-GFP
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SLC45A1
  • Species
    H. sapiens (human)
  • Entrez Gene
    SLC45A1 (a.k.a. DNB5, IDDNPF, PAST-A)
  • Tag / Fusion Protein
    • 3xFLAG-EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMX-Flag-GFP_SLC45A1 was a gift from Alessandro Ori (Addgene plasmid # 227951 ; http://n2t.net/addgene:227951 ; RRID:Addgene_227951)

  • For your References section:

    Cell-type resolved protein atlas of brain lysosomes identifies SLC45A1-associated disease as a lysosomal disorder. Ghoochani A, Heiby JC, Rawat ES, Medoh UN, Di Fraia D, Dong W, Gastou M, Rastogi M, Hernandez V, Nyame K, Laqtom NN, Durso W, Valkova C, Isakova A, Kaether C, Wernig M, Gomez-Ospina N, Franke C, Ori A, Abu-Remaileh M. Cell. 2026 Feb 5;189(3):765-782.e31. doi: 10.1016/j.cell.2025.12.012. Epub 2026 Jan 22. 10.1016/j.cell.2025.12.012 PubMed 41576950
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