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JL054: pPB_NFκB::GFP-GEMINI(A)-P1N4_TRE::HaloTag-GEMINI(A)_UbC::rtTA3-IRES-PuroR
(Plasmid #228884)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228884 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pPiggyBac
  • Total vector size (bp) 12219
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP-fused GEMINI(A), HaloTag-fuse GEMINI(A)
  • Alt name
    GFP-GEMINI(A); HT-GEMINI(A)
  • Species
    Synthetic
  • Insert Size (bp)
    3900
  • Promoter pNFκB; TRE

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.07.16.664392 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JL054: pPB_NFκB::GFP-GEMINI(A)-P1N4_TRE::HaloTag-GEMINI(A)_UbC::rtTA3-IRES-PuroR was a gift from Dingchang Lin (Addgene plasmid # 228884 ; http://n2t.net/addgene:228884 ; RRID:Addgene_228884)

  • For your References section:

    Genetically encoded assembly recorder temporally resolves cellular histories in cellulo and in vivo. Yan Y, Lu J, Li Z, Zhao Z, Shay TF, Wang S, Lei Y, Wang Y, Chen W, Parker P, Yang H, Qi A, Sun Y, Bergles DE, Baker D, Lin D. bioRxiv 2025.07.16.664392 10.1101/2025.07.16.664392
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