pET28a-UBC9
(Plasmid
#228949)
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Purposeexpress a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228949 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a(+)
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5236
- Total vector size (bp) 5758
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameUBC9
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Alt nameUBE2I
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SpeciesH. sapiens (human)
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Insert Size (bp)522
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GenBank IDNM_194260.3
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Entrez GeneUBE2I (a.k.a. C358B7.1, P18, UBC9)
- Promoter T7-lac
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Tag
/ Fusion Protein
- His tag and thrombine cleavage site : MGHHHHHHSSGLVPRGS (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-UBC9 was a gift from Marcin Suskiewicz (Addgene plasmid # 228949 ; http://n2t.net/addgene:228949 ; RRID:Addgene_228949)