pET28a-UBC9
(Plasmid #228949)

• Purpose: express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)
• Depositing Lab: Marcin Suskiewicz
• Publication: Production and purification of SUMO-UBC9 and SUMO-RANGAP1CTD (unpublished)

Backbone

• Vector backbone: pET28a(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5236
• Total vector size (bp): 5758
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: UBC9
• Alt name: UBE2I
• Species: H. sapiens (human)
• Insert Size (bp): 522
• GenBank ID: NM_194260.3
• Entrez Gene: UBE2I (a.k.a. C358B7.1, P18, UBC9)
• Promoter: T7-lac
• Tag / Fusion Protein:
  • His tag and thrombine cleavage site : MGHHHHHHSSGLVPRGS (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry