pJP_Osc05
(Plasmid
#229040)
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PurposeLight inducible three-node circuit that can generate ring patterns in a growing bacterial colony upon blue light pulses. Heterologous expression of dCas9, Csy4, and VVD-AraC are necessary.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 229040 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepJ2072.2_CRISPRlator
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Backbone manufacturerYolanda Schaerli, Addgene plasmid #140674
- Backbone size w/o insert (bp) 6900
- Total vector size (bp) 7234
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Modifications to backboneThe Kanamycin resistance gene was inserted between mCitrine and mCerulean. pBAD promoter controls the expression of mCherry node.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepBAD promoter
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Insert Size (bp)285
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.12.18.629005 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJP_Osc05 was a gift from Yolanda Schaerli (Addgene plasmid # 229040 ; http://n2t.net/addgene:229040 ; RRID:Addgene_229040)
Map uploaded by the depositor.
