pCDF_AtRaf1/Raf2/RbcX2/BSD2/RbcX1
(Plasmid #229510)

• Purpose: Expresses Arabidopsis thaliana chaperones: Raf1,Raf2,RbcX2,BSD2,RbcX1
• Depositing Lab: Laura Gunn
• Publication: Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849. doi: 10.1016/j.molp.2024.10.013. Epub 2024 Nov 2.

Backbone

• Vector backbone: pCDFDuet-1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3781
• Total vector size (bp): 7587
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Streptomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Rubisco accumulation factor 1
• Alt name: Raf1
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1176
• Mutation: N terminus chloroplast transit peptide truncation
• Entrez Gene: AT3G04550 (a.k.a. AT3G04550, F7O18.2, F7O18_2)
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: Not available

Gene/Insert 2

• Gene/Insert name: Rubisco accumulation factor 2
• Alt name: Raf2
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 519
• Mutation: N terminus chloroplast transit peptide truncation
• Entrez Gene: AT5G51110 (a.k.a. AT5G51110, MWD22.5, MWD22_5)
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: Not available

Gene/Insert 3

• Gene/Insert name: RbcX2
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 429
• Mutation: N terminus chloroplast transit peptide truncation
• Entrez Gene: RbcX2 (a.k.a. AT5G19855, AtRbcX2, homologue of cyanobacterial RbcX 2)
• Promoter: T7

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: Not available

Gene/Insert 4

• Gene/Insert name: BSD2
• Alt name: BUNDLE SHEATH DEFECTIVE 2
• Insert Size (bp): 249
• Mutation: N terminus chloroplast transit peptide truncation
• GenBank ID: AEE78314.1
• Promoter: T7

Cloning Information for Gene/Insert 4

• Cloning method: Unknown
• 5′ sequencing primer: Not Available

Gene/Insert 5

• Gene/Insert name: RbcX1
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 387
• Mutation: N terminus chloroplast transit peptide truncation
• GenBank ID: AY142573.1
• Promoter: T7

Cloning Information for Gene/Insert 5

• Cloning method: Unknown
• 5′ sequencing primer: Not Available

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Gene Synthesis from Genscript. Design of the Rubisco assembly factor inserts was mirroring pAtR1/R2/RX/B2 as shown in Aigner et al 2017 (10.1126/science.aap9221), with the addition of RbcX isoform, RbcXI after the BSD2 gene.