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(Plasmid #23013)

Full plasmid sequence is not available for this item.



Item Catalog # Description Quantity Price (USD)
Plasmid 23013 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 8900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Propagate at 30 degrees Celsius and 5ug/mL tetracylcine.
  • Copy number
    Low Copy


  • Gene/Insert name
    I-CreI homing endonuclease
  • Species
    Chlamydomonas reinhardtii
  • Insert Size (bp)
  • Mutation
    I-CreI expression is controlled by the arabinose promoter.
  • GenBank ID

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site PvuII (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    I derived code for I-CreI behind the arabinose promoter and the arabinose regulatory elements from plasmid pAE from Lenny Seligman of Pomona College, an author on the cited paper describing the plasmid.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Seligman, LM et al., 1997, Genetics, 147: 1653-1664

The plasmid can be propagated in 0.2% glucose to keep expression levels low and to guard against mutations.

Addgene's quality control sequence shows several sequence discrepancies with the available NCBI reference sequence for AraC and ICreI. Depositor tested this plasmid and found the plasmid can be used to cure cells of other plasmids that contain an I-CreI cut site, and the pCURE can be removed from cells via its temperature sensitive origin of replication. Hence, these mutations are not detrimental to the plasmid's function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCURE was a gift from James Bowie (Addgene plasmid # 23013 ; ; RRID:Addgene_23013)
  • For your References section:

    Genetic selection system for improving recombinant membrane protein expression in E. coli. Massey-Gendel E, Zhao A, Boulting G, Kim HY, Balamotis MA, Seligman LM, Nakamoto RK, Bowie JU. Protein Sci. 2009 Feb . 18(2):372-83. 10.1002/pro.39 PubMed 19165721
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