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rag2:myr-Akt2_pISceI
(Plasmid #231495)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231495 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pISceI
  • Vector type
    Zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Akt2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Akt2 (a.k.a. 2410016A19Rik, PKB, PKBbeta)
  • Tag / Fusion Protein
    • N-terminal myristolation signal (N terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    A. Thomas Look Laboratory, Dana-Farber Cancer Institute

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rag2:myr-Akt2_pISceI was a gift from Alejandro Gutierrez (Addgene plasmid # 231495 ; http://n2t.net/addgene:231495 ; RRID:Addgene_231495)

  • For your References section:

    A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation. Ali RH, Orellana EA, Lee SH, Chae YC, Chen Y, Clauwaert J, Kennedy AL, Gutierrez AE, Papke DJ, Valenzuela M, Silverman B, Falzetta A, Ficarro SB, Marto JA, Fletcher CDM, Perez-Atayde A, Alcindor T, Shimamura A, Prensner JR, Gregory RI, Gutierrez A. Mol Cell. 2025 Mar 6;85(5):948-961.e11. doi: 10.1016/j.molcel.2025.01.003. Epub 2025 Jan 31. 10.1016/j.molcel.2025.01.003 PubMed 39892392
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