pUC57-FlpTag-GFP-ph2
(Plasmid #231899)

• Purpose: Vector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 2.
• Depositing Lab: Alexander Borst
• Publication: Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.7554/eLife.62953.

Backbone

• Vector backbone: pFlip-Flop-P0
• Backbone size w/o insert (bp): 5285
• Total vector size (bp): 4107
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SD-GFP-SA
• Species: Synthetic
• Insert Size (bp): 991

Resource Information

• Supplemental Documents:
  • pUC57-FlpTag-GFP-ph2.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The FlpTag insertion consists of an inverted sequence containing a splice acceptor (SA), GFP, and a splice donor (SD), flanked on either side by FRT and FRT14 recombination sites. This configuration allows conditional protein tagging, where the cassette orientation is controlled by Flp recombinase, enabling specific expression of GFP in targeted cells or tissues. FlpTag is integrated into the genome at specific attP sites, targeting the gene of interest through phiC31 integrase-mediated recombination at the designated gene locus.