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Addgene

pET-Hia5-OPT1
(Plasmid #231968)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 231968 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET30a+
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5422
  • Total vector size (bp) 5422
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Luria Broth with kanamycin
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DNA adenine N6-methyltransferase Hia5
  • Alt name
    Hia5
  • Insert Size (bp)
    843
  • Tag / Fusion Protein
    • Histidine tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-Hia5-OPT1 was a gift from Schahram Akbarian (Addgene plasmid # 231968 ; http://n2t.net/addgene:231968 ; RRID:Addgene_231968)

  • For your References section:

    Single chromatin fiber profiling and nucleosome position mapping in the human brain. Peter CJ, Agarwal A, Watanabe R, Kassim BS, Wang X, Lambert TY, Javidfar B, Evans V, Dawson T, Fridrikh M, Girdhar K, Roussos P, Nageshwaran SK, Tsankova NM, Sebra RP, Vollger MR, Stergachis AB, Hasson D, Akbarian S. Cell Rep Methods. 2024 Nov 25:100911. doi: 10.1016/j.crmeth.2024.100911. 10.1016/j.crmeth.2024.100911 PubMed 39631398
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