RUBYm2
(Plasmid
#232175)
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PurposeIntroduce a C-to-T mutation to generate an early stop codon (Q139 to stop), thereby interrupting RUBY expression
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 232175 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepHDE
- Total vector size (bp) 14335
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRUBY mutant2 and CaMV 35S promoter
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SpeciesSynthetic; Cauliflower mosaic virus
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Insert Size (bp)4892
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RUBYm2 was a gift from Yiping Qi (Addgene plasmid # 232175 ; http://n2t.net/addgene:232175 ; RRID:Addgene_232175) -
For your References section:
Seeing is believing: Genome editing made easy with RUBY for educational purposes. Sexton WK, Cheng Y, Yang B, Ullah H, Qi Y, Xiao S. Plant Physiol. 2025 Jul 3;198(3):kiaf265. doi: 10.1093/plphys/kiaf265. 10.1093/plphys/kiaf265 PubMed 40696509