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Addgene

RUBY-ABE#2
(Plasmid #232176)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232176 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pYPQ166-ABE8e
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    2x35s-ecTadA8e-SpCas9-D10A-AtU3-sgRNA-mis4-gRNA scaffold
  • Tags / Fusion Proteins
    • NLS
    • 3xflag

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Addgene NGS result differs slightly from the depositor's provided sequence: 2bp and a 1bp indel in 2x35S promoter. The depositor notes that this is not of concern for the plasmid's function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RUBY-ABE#2 was a gift from Yiping Qi (Addgene plasmid # 232176 ; http://n2t.net/addgene:232176 ; RRID:Addgene_232176)

  • For your References section:

    Seeing is believing: Genome editing made easy with RUBY for educational purposes. Sexton WK, Cheng Y, Yang B, Ullah H, Qi Y, Xiao S. Plant Physiol. 2025 Jul 3;198(3):kiaf265. doi: 10.1093/plphys/kiaf265. 10.1093/plphys/kiaf265 PubMed 40696509
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