nlsSalicS1-NR
(Plasmid
#232192)
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PurposeNegative control for nlsSalicS1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 232192 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backboneGolden gate level 2 plant expression background position 2
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Backbone manufacturerMoClo
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Vector typePlant Expression
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Selectable markersKanamycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenlsSalicS1-NR
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SpeciesSynthetic
- Promoter P16
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Tags
/ Fusion Proteins
- His-tag (N terminal on insert)
- Myc (C terminal on insert)
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
nlsSalicS1-NR was a gift from Alexander Jones (Addgene plasmid # 232192 ; http://n2t.net/addgene:232192 ; RRID:Addgene_232192) -
For your References section:
SALICYLIC ACID SENSOR1 reveals the propagation of an SA hormone surge during plant pathogen advance. Tang B, Lu J, Leontovycova H, Hoffmann G, Rowe JH, O'Donnell SF, Grange-Guermente M, Larsen B, Wimalasekera R, Carella P, Incarbone M, Kalachova T, Jones AM. Science. 2025 Oct 9;390(6769):188-194. doi: 10.1126/science.adw7650. Epub 2025 Oct 9. 10.1126/science.adw7650 PubMed 41066584