pAAV2/2
(Plasmid #232199)

• Purpose: AAV packaging plasmid expressing AAV2 Rep and capsid proteins
• Depositing Labs: Melina Fan, Addgene Research Program
• Publication: Addgene AAV RepCap Plasmids (unpublished)

Backbone

• Vector backbone: pUC19 with additional elements
• Total vector size (bp): 7406
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rep2/Cap2
• Species: Parvovirus

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13 Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

Addgene recommends users replace Addgene #104963 with Addgene #232199, as it consistently yields higher concentrations. Please be advised that high titers of AAV2 promote aggregation. When transitioning from Addgene #104963 to Addgene #232199, Addgene suggests increasing your final elution volume to reduce aggregation associated with a high concentration. The optimal elution volume must be empirically determined.