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pIRES-mCerulean-ABI-RhoA/N12-RhoA/13C(Q63L)-PYL-mVenus-CAAX
(Plasmid #232266)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232266 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pIRES
  • Backbone manufacturer
    Takara Bio
  • Backbone size w/o insert (bp) 6105
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    mCerulean-ABI-RhoA/N12
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1728
  • Mutation
    RhoA-Q63L
  • Entrez Gene
    RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCerulean (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    RhoA/13C(Q63L)-PYL-mVenus-CAAX
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1902
  • Mutation
    RhoA-Q63L
  • Entrez Gene
    RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
  • Promoter CMV
  • Tag / Fusion Protein
    • mVenus (C terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid will express two separate inserts, which include split-GTPase fragments that are fused with different CIDs and FPs

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES-mCerulean-ABI-RhoA/N12-RhoA/13C(Q63L)-PYL-mVenus-CAAX was a gift from Cliff Stains (Addgene plasmid # 232266 ; http://n2t.net/addgene:232266 ; RRID:Addgene_232266)

  • For your References section:

    Split-Small GTPase Reassembly as a Method to Control Cellular Signaling with User-Defined Inputs. He Y, Faulkner BM, Weatherford RS, Hyun E, Stains CI. ACS Chem Biol. 2025 Aug 26. doi: 10.1021/acschembio.5c00083. 10.1021/acschembio.5c00083 PubMed 40856289
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