pEN_TT PCSK5(sgRR,T288P)-V5
(Plasmid
#232451)
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PurposeGateway entry vector for an inducible sg09 resistant PCSK5_T288P mutant
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 232451 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepEN_TTmiRC2
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Backbone manufacturerIain Fraser, Addgene plasmid #25752
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Vector typeGateway entry vector
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePCSK5(T288P)
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Alt namePC5
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Alt namePC6
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SpeciesH. sapiens (human)
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Mutationa75g_t84c_g87c; T288P
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Entrez GenePCSK5 (a.k.a. PC5, PC6, PC6A, SPC6)
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Unknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEN_TT PCSK5(sgRR,T288P)-V5 was a gift from Kevin Janes (Addgene plasmid # 232451 ; http://n2t.net/addgene:232451 ; RRID:Addgene_232451) -
For your References section:
PCSK5M452I is a recessive hypomorph exclusive to MCF10DCIS.com cells. Marohl T, Atkins KA, Wang L, Janes KA. Mol Cancer Res. 2025 Oct 28. doi: 10.1158/1541-7786.MCR-25-0211. 10.1158/1541-7786.MCR-25-0211 PubMed 41148168