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pSLIK PCSK5(sgRR,T288P)-V5 hygro
(Plasmid #232454)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232454 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pSLIK_Hygro
  • Backbone manufacturer
    Iain Fraser, Addgene plasmid #25737
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PCSK5(T288P)
  • Alt name
    PC5
  • Alt name
    PC6
  • Species
    H. sapiens (human)
  • Mutation
    a75g_t84c_g87c; T288P
  • Entrez Gene
    PCSK5 (a.k.a. PC5, PC6, PC6A, SPC6)
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSLIK PCSK5(sgRR,T288P)-V5 hygro was a gift from Kevin Janes (Addgene plasmid # 232454 ; http://n2t.net/addgene:232454 ; RRID:Addgene_232454)

  • For your References section:

    PCSK5M452I is a recessive hypomorph exclusive to MCF10DCIS.com cells. Marohl T, Atkins KA, Wang L, Janes KA. Mol Cancer Res. 2025 Oct 28. doi: 10.1158/1541-7786.MCR-25-0211. 10.1158/1541-7786.MCR-25-0211 PubMed 41148168
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