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EDCPV PCSK5_sg09
(Plasmid #232455)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232455 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    EDCPV
  • Backbone manufacturer
    Raffaella Sordella, Addgene plasmid #90085
  • Vector type
    Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PCSK5 sgRNA
  • Alt name
    PC5
  • Alt name
    PC6
  • gRNA/shRNA sequence
    CTACACGGGAAAGAACATTGTGG
  • Species
    H. sapiens (human)
  • Entrez Gene
    PCSK5 (a.k.a. PC5, PC6, PC6A, SPC6)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EDCPV PCSK5_sg09 was a gift from Kevin Janes (Addgene plasmid # 232455 ; http://n2t.net/addgene:232455 ; RRID:Addgene_232455)

  • For your References section:

    PCSK5M452I is a recessive hypomorph exclusive to MCF10DCIS.com cells. Marohl T, Atkins KA, Wang L, Janes KA. Mol Cancer Res. 2025 Oct 28. doi: 10.1158/1541-7786.MCR-25-0211. 10.1158/1541-7786.MCR-25-0211 PubMed 41148168
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