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Addgene

pAAV-CAG-DIO-NES-SomaFRCaMPi
(Plasmid #232836)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232836 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAAV
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NES-FRCaMPi
  • Species
    Synthetic
  • Insert Size (bp)
    2085
  • Entrez Gene
    NES (a.k.a. Nbla00170)
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer GTTATACTAGAATTCGGTACCTAAGCCACCATGCTTCAACTTCCTCCTCTTGAACG
  • 3′ sequencing primer AGGGATCCCCTTACTTGTACActaatacagacgctggggcttgcccatg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CAG-DIO-NES-SomaFRCaMPi was a gift from Kiryl Piatkevich (Addgene plasmid # 232836 ; http://n2t.net/addgene:232836 ; RRID:Addgene_232836)

  • For your References section:

    A sensitive soma-localized red fluorescent calcium indicator for in vivo imaging of neuronal populations at single-cell resolution. Zhou S, Zhu Q, Eom M, Fang S, Subach OM, Ran C, Alvarado JS, Sunkavalli PS, Dong Y, Wang Y, Hu J, Zhang H, Wang Z, Sun X, Yang T, Mu Y, Yoon YG, Guo ZV, Subach FV, Piatkevich KD. PLoS Biol. 2025 Apr 29;23(4):e3003048. doi: 10.1371/journal.pbio.3003048. eCollection 2025 Apr. 10.1371/journal.pbio.3003048 PubMed 40299972
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