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J364B_AttB_mkozak_STIM1_IRES_CaMPARI2-P2A-PuroR
(Plasmid #232939)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 232939 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    Bxb1 attB recombination vector
  • Backbone manufacturer
    Matreyek Lab
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    STIM1
  • Species
    H. sapiens (human)
  • Entrez Gene
    STIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)

Gene/Insert 2

  • Gene/Insert name
    CaMPARI2
  • Species
    Synthetic

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    STIM1; pLNCX2-STIM1 was a gift from Shengyu Yang (Addgene plasmid # 89817 ; http://n2t.net/addgene:89817 ; RRID:Addgene_89817) CaMPARI2; pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 was a gift from Eric Schreiter (Addgene plasmid # 101060 ; http://n2t.net/addgene:101060 ; RRID:Addgene_101060)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.01.23.634525 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    J364B_AttB_mkozak_STIM1_IRES_CaMPARI2-P2A-PuroR was a gift from Kenneth Matreyek (Addgene plasmid # 232939 ; http://n2t.net/addgene:232939 ; RRID:Addgene_232939)

  • For your References section:

    Comprehensive mutational characterization of the calcium-sensing STIM1 EF-hand reveals residues essential for structure and function. Kamath ND, Matreyek KA. Genetics. 2025 Jul 30:iyaf146. doi: 10.1093/genetics/iyaf146. 10.1093/genetics/iyaf146 PubMed 40737484
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