pAAV-CMV-LNI-bGH
(Plasmid #233203)

• Purpose: AAV expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogramming
• Depositing Lab: Kate Galloway
• Publication: Wang et al Cell Syst. 2025 Mar 10, Volume 16, Issue 4 :101206. doi: 10.1016/j.cels.2025.101206

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Backbone

• Vector backbone: AAV2
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mLhx3-P2A-mNgn2x3HA-T2A-Isl1
• Species: M. musculus (mouse)
• Entrez Gene: Neurog2 (a.k.a. Atoh4, Math4A, bHLHa8, ngn-2, ngn2)
• Entrez Gene: Lhx3 (a.k.a. Lim3, P-LIM, mLIM3, mLim-3)
• Entrez Gene: Isl1

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Supplemental Documents:
  • pAAV-CMV-LNI-bGH.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry